rat multiple tissue northern blots Search Results


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c6 rat glioma cells  (ATCC)
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ATCC c6 rat glioma cells
Figure 5. Multimodality imaging of the tumor xenografts expressing mtfl-mrfp1-wttk and fl-mrfp1-wttk fusion reporter genes by bioluminescence and microPET imaging in living mice. A, bioluminescence imaging of a living mouse implanted with <t>C6</t> tumors stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes: 5 106 C6 cells stably expressing either CMV-mtfl-mrfp1-wttk fusion (A) or CMV-fl-mrfp1-wttk fusion (B) were implanted on two shoulders of a nude mouse and the tumors were allowed to reach a diameter of 6 to 8 mm. The mouse was injected with D-luciferin (100 Ag/mouse) and imaged for bioluminescence. The mtfl-mrfp1-wttk tumor showed higher luciferase signal (P = NS) than the fl-mrfp1-wttk fusion tumors. B, microPET imaging of a living mouse implanted with tumor-expressing C6 cells stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes for 18F-FEAU and 18F-FHBG uptake: the same mouse described in (A) was first injected with f200 ACi 18F-FEAU, and microPET imaging was done after 1 h for 10 min. Specific uptake of 18F-FEAU was seen at both (A and B) tumors (P = NS) with very low activity in the gastrointestinal tract (GI). After 24 h, the mouse was again injected with f200 ACi 18F-FHBG observed in both tumors (A and B) with high nonspecific accumulation in the gastrointestinal tract. C, graphical representation of the bioluminescence signal exhibited by the mtfl-mrfp1-wttk (A) and fl-mrfp1-wttk (B) tumors of six nude mice. Bioluminescence signals were calculated for the respective ROIs drawn over the sites of tumors (A and B). Representative of six experiments. Columns, (p/s/cm2/sr) maximum 105; bars, SE. D, comparison between the uptake of 18F-FHBG by tumors expressing mtfl-mrfp1wttk and fl-mrfp1-wttk genes in six nude mice. % ID/g of 18F-FEAU and 18F-FHBG uptakes were calculated for the respective ROIs drawn over the tumors. (A and B) showed similar uptake for both tracers (P = NS). However, 18F-FEAU accumulation was significantly higher than 18F-FHBG (P > 0.05) accumulation.
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goat anti rat ig  (Vector Laboratories)
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Vector Laboratories goat anti rat ig
Figure 5. Multimodality imaging of the tumor xenografts expressing mtfl-mrfp1-wttk and fl-mrfp1-wttk fusion reporter genes by bioluminescence and microPET imaging in living mice. A, bioluminescence imaging of a living mouse implanted with <t>C6</t> tumors stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes: 5 106 C6 cells stably expressing either CMV-mtfl-mrfp1-wttk fusion (A) or CMV-fl-mrfp1-wttk fusion (B) were implanted on two shoulders of a nude mouse and the tumors were allowed to reach a diameter of 6 to 8 mm. The mouse was injected with D-luciferin (100 Ag/mouse) and imaged for bioluminescence. The mtfl-mrfp1-wttk tumor showed higher luciferase signal (P = NS) than the fl-mrfp1-wttk fusion tumors. B, microPET imaging of a living mouse implanted with tumor-expressing C6 cells stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes for 18F-FEAU and 18F-FHBG uptake: the same mouse described in (A) was first injected with f200 ACi 18F-FEAU, and microPET imaging was done after 1 h for 10 min. Specific uptake of 18F-FEAU was seen at both (A and B) tumors (P = NS) with very low activity in the gastrointestinal tract (GI). After 24 h, the mouse was again injected with f200 ACi 18F-FHBG observed in both tumors (A and B) with high nonspecific accumulation in the gastrointestinal tract. C, graphical representation of the bioluminescence signal exhibited by the mtfl-mrfp1-wttk (A) and fl-mrfp1-wttk (B) tumors of six nude mice. Bioluminescence signals were calculated for the respective ROIs drawn over the sites of tumors (A and B). Representative of six experiments. Columns, (p/s/cm2/sr) maximum 105; bars, SE. D, comparison between the uptake of 18F-FHBG by tumors expressing mtfl-mrfp1wttk and fl-mrfp1-wttk genes in six nude mice. % ID/g of 18F-FEAU and 18F-FHBG uptakes were calculated for the respective ROIs drawn over the tumors. (A and B) showed similar uptake for both tracers (P = NS). However, 18F-FEAU accumulation was significantly higher than 18F-FHBG (P > 0.05) accumulation.
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Image Search Results


Figure 5. Multimodality imaging of the tumor xenografts expressing mtfl-mrfp1-wttk and fl-mrfp1-wttk fusion reporter genes by bioluminescence and microPET imaging in living mice. A, bioluminescence imaging of a living mouse implanted with C6 tumors stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes: 5 106 C6 cells stably expressing either CMV-mtfl-mrfp1-wttk fusion (A) or CMV-fl-mrfp1-wttk fusion (B) were implanted on two shoulders of a nude mouse and the tumors were allowed to reach a diameter of 6 to 8 mm. The mouse was injected with D-luciferin (100 Ag/mouse) and imaged for bioluminescence. The mtfl-mrfp1-wttk tumor showed higher luciferase signal (P = NS) than the fl-mrfp1-wttk fusion tumors. B, microPET imaging of a living mouse implanted with tumor-expressing C6 cells stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes for 18F-FEAU and 18F-FHBG uptake: the same mouse described in (A) was first injected with f200 ACi 18F-FEAU, and microPET imaging was done after 1 h for 10 min. Specific uptake of 18F-FEAU was seen at both (A and B) tumors (P = NS) with very low activity in the gastrointestinal tract (GI). After 24 h, the mouse was again injected with f200 ACi 18F-FHBG observed in both tumors (A and B) with high nonspecific accumulation in the gastrointestinal tract. C, graphical representation of the bioluminescence signal exhibited by the mtfl-mrfp1-wttk (A) and fl-mrfp1-wttk (B) tumors of six nude mice. Bioluminescence signals were calculated for the respective ROIs drawn over the sites of tumors (A and B). Representative of six experiments. Columns, (p/s/cm2/sr) maximum 105; bars, SE. D, comparison between the uptake of 18F-FHBG by tumors expressing mtfl-mrfp1wttk and fl-mrfp1-wttk genes in six nude mice. % ID/g of 18F-FEAU and 18F-FHBG uptakes were calculated for the respective ROIs drawn over the tumors. (A and B) showed similar uptake for both tracers (P = NS). However, 18F-FEAU accumulation was significantly higher than 18F-FHBG (P > 0.05) accumulation.

Journal: Cancer Research

Article Title: Construction and Validation of Improved Triple Fusion Reporter Gene Vectors for Molecular Imaging of Living Subjects

doi: 10.1158/0008-5472.can-06-2402

Figure Lengend Snippet: Figure 5. Multimodality imaging of the tumor xenografts expressing mtfl-mrfp1-wttk and fl-mrfp1-wttk fusion reporter genes by bioluminescence and microPET imaging in living mice. A, bioluminescence imaging of a living mouse implanted with C6 tumors stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes: 5 106 C6 cells stably expressing either CMV-mtfl-mrfp1-wttk fusion (A) or CMV-fl-mrfp1-wttk fusion (B) were implanted on two shoulders of a nude mouse and the tumors were allowed to reach a diameter of 6 to 8 mm. The mouse was injected with D-luciferin (100 Ag/mouse) and imaged for bioluminescence. The mtfl-mrfp1-wttk tumor showed higher luciferase signal (P = NS) than the fl-mrfp1-wttk fusion tumors. B, microPET imaging of a living mouse implanted with tumor-expressing C6 cells stably expressing the mtfl-mrfp1-wttk and fl-mrfp1-wttk triple fusion reporter genes for 18F-FEAU and 18F-FHBG uptake: the same mouse described in (A) was first injected with f200 ACi 18F-FEAU, and microPET imaging was done after 1 h for 10 min. Specific uptake of 18F-FEAU was seen at both (A and B) tumors (P = NS) with very low activity in the gastrointestinal tract (GI). After 24 h, the mouse was again injected with f200 ACi 18F-FHBG observed in both tumors (A and B) with high nonspecific accumulation in the gastrointestinal tract. C, graphical representation of the bioluminescence signal exhibited by the mtfl-mrfp1-wttk (A) and fl-mrfp1-wttk (B) tumors of six nude mice. Bioluminescence signals were calculated for the respective ROIs drawn over the sites of tumors (A and B). Representative of six experiments. Columns, (p/s/cm2/sr) maximum 105; bars, SE. D, comparison between the uptake of 18F-FHBG by tumors expressing mtfl-mrfp1wttk and fl-mrfp1-wttk genes in six nude mice. % ID/g of 18F-FEAU and 18F-FHBG uptakes were calculated for the respective ROIs drawn over the tumors. (A and B) showed similar uptake for both tracers (P = NS). However, 18F-FEAU accumulation was significantly higher than 18F-FHBG (P > 0.05) accumulation.

Article Snippet: Chinese hamster ovary (CHO) cancer cells, 293T human embryonic kidney cells, MDA-MB human breast cancer cells, and C6 rat glioma cells were used (American Type Culture Collection, Manassas, VA).

Techniques: Imaging, Expressing, Stable Transfection, Injection, Luciferase, Activity Assay, Comparison